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Covalently crosslinked chitosan hydrogel: Properties of in vitro degradation and chondrocyte encapsulation

Paper ID Volume ID Publish Year Pages File Format Full-Text
2563 116 2007 9 PDF Available
Title
Covalently crosslinked chitosan hydrogel: Properties of in vitro degradation and chondrocyte encapsulation
Abstract

In vitro degradation and chondrocyte-encapsulation of chitosan hydrogel made of crosslinkable and water-soluble chitosan derivative (CML) at neutral pH and body temperature were studied with respect to weight loss, cytoviability, DNA content and cell morphology. In vitro degradation of the chitosan hydrogels was sensitive to their crosslinking degree and existence of lysozyme in the solution. Chitosan hydrogel (Gel-I5) fabricated from 1% CML and 5 mM ammonium persulfate (APS)/N,N,N′,N′-tetramethylethylenediamine (TMEDA) displayed no degradation in phosphate buffered saline (PBS) after 18 d, but degraded completely at 8 d in 1 mg/ml lysozyme/PBS. The chitosan hydrogel fabricated from 10 mM APS/TMEDA was non-degradable even in lysozyme/PBS solution after 18 d. The hydrogel loaded with chondrocytes in cell culture medium, however, was susceptible to degradation during the in vitro culture. In vitro culture of the encapsulated chondrocytes in the chitosan hydrogel demonstrated that the cells retained round shaped morphology and could survive through a 12 d-culture period, although the DNA assay detected an overall reduction of the cell number. These features provide a great opportunity to use the chitosan hydrogel as an injectable scaffold in tissue engineering and orthopaedics.

Keywords
Chitosan; Hydrogel; Degradation; Chondrocytes; Injectable scaffold
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Covalently crosslinked chitosan hydrogel: Properties of in vitro degradation and chondrocyte encapsulation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 3, Issue 1, January 2007, Pages 23–31
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us