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The effect of intracellular ppGpp levels on glutamate and lysine overproduction in Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
25631 43586 2006 10 PDF Available
Title
The effect of intracellular ppGpp levels on glutamate and lysine overproduction in Escherichia coli
Abstract

Although the enhancement of amino-acid synthesis by guanosine-3′,5′-tetraphosphate (ppGpp) is well known, the effect of intracellular ppGpp levels on amino-acid overproduction in Escherichia coli has not been investigated. In this study, we demonstrate that overexpression of the relA gene, encoding ppGpp synthetase, increases the accumulation of amino acids, such as glutamate and lysine, in amino-acid-overproducing strains of E. coli. Elevation of intracellular ppGpp levels due to depletion of required amino acids also enhances glutamate overproduction. Moreover, the extent of overproduction is highly dependent on the intracellular ppGpp level. These results demonstrate that amino-acid overproduction in E. coli is closely connected to amino-acid auxotrophy via the accumulation of ppGpp.

Keywords
OD, optical density; ppGpp, guanosine-3′,5′-tetraphosphate; rRNA, ribosomal RNA; tRNA, transfer RNAEscherichia coli; ppGpp; Stringent response; relA; l-glutamate; l-lysine
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The effect of intracellular ppGpp levels on glutamate and lysine overproduction in Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 125, Issue 3, 18 September 2006, Pages 328–337
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us