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Automated DNA extraction from genetically modified maize using aminosilane-modified bacterial magnetic particles

Paper ID Volume ID Publish Year Pages File Format Full-Text
25634 43586 2006 8 PDF Available
Title
Automated DNA extraction from genetically modified maize using aminosilane-modified bacterial magnetic particles
Abstract

A novel, automated system, PNE-1080, equipped with eight automated pestle units and a spectrophotometer was developed for genomic DNA extraction from maize using aminosilane-modified bacterial magnetic particles (BMPs). The use of aminosilane-modified BMPs allowed highly accurate DNA recovery. The (A260–A320):(A280–A320) ratio of the extracted DNA was 1.9 ± 0.1. The DNA quality was sufficiently pure for PCR analysis. The PNE-1080 offered rapid assay completion (30 min) with high accuracy. Furthermore, the results of real-time PCR confirmed that our proposed method permitted the accurate determination of genetically modified DNA composition and correlated well with results obtained by conventional cetyltrimethylammonium bromide (CTAB)-based methods.

Keywords
Aminosilane-modified bacterial magnetic particles; DNA extraction; Genetically modified maize
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Automated DNA extraction from genetically modified maize using aminosilane-modified bacterial magnetic particles
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 125, Issue 3, 18 September 2006, Pages 361–368
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us