fulltext.study @t Gmail

In situ detoxification and continuous cultivation of dilute-acid hydrolyzate to ethanol by encapsulated S. cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
25636 43586 2006 8 PDF Available
Title
In situ detoxification and continuous cultivation of dilute-acid hydrolyzate to ethanol by encapsulated S. cerevisiae
Abstract

Dilute-acid lignocellulosic hydrolyzate was successfully fermented to ethanol by encapsulated Saccharomyces cerevisiae at dilution rates up to 0.5 h−1. The hydrolyzate was so toxic that freely suspended yeast cells could ferment it continuously just up to dilution rate 0.1 h−1, where the cells lost 75% of their viability measured by colony forming unit (CFU). However, encapsulation increased their capacity for in situ detoxification of the hydrolyzate and protected the cells against the inhibitors present in the hydrolyzate. While the cells were encapsulated, they could successfully ferment the hydrolyzate at tested dilution rates 0.1–0.5 h−1, and keep more than 75% cell viability in the worst conditions. They produced ethanol with yield 0.44 ± 0.01 g/g and specific productivity 0.14–0.17 g/(g h) at all dilution rates. Glycerol was the main by-product of the cultivations, which yielded 0.039–0.052 g/g. HMF present in the hydrolyzate was converted 48–71% by the encapsulated yeast, while furfural was totally converted at dilution rates 0.1 and 0.2 h−1 and partly at the higher rates. Continuous cultivation of encapsulated yeast was also investigated on glucose in synthetic medium up to dilution rate 1.0 h−1. At this highest rate, ethanol and glycerol were also the major products with yields 0.43 and 0.076 g/g, respectively. The experiments lasted for 18–21 days, and no damage in the capsules was detected.

Keywords
Encapsulation; S. cerevisiae; Ethanol; In situ detoxification; Dilute-acid hydrolyzate
First Page Preview
In situ detoxification and continuous cultivation of dilute-acid hydrolyzate to ethanol by encapsulated S. cerevisiae
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 125, Issue 3, 18 September 2006, Pages 377–384
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us