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Chemically surface modified gel (CSMG): An excellent enzyme-immobilization matrix for industrial processes

Paper ID Volume ID Publish Year Pages File Format Full-Text
25638 43586 2006 13 PDF Available
Title
Chemically surface modified gel (CSMG): An excellent enzyme-immobilization matrix for industrial processes
Abstract

Invertase from S. cerevisiae has been immobilized on porous silica matrix, formed using sol–gel chemistry, with surface area of approximately 650 m2/g. The co-condensation of silica sol with 3-aminopropyl(triethoxy)silane produced an amino-chemically surface modified silica gel (N-CSMG) with a very high ligand loading of 3.6 mmol/g SiO2; significantly higher than commercially available matrices. Surface amine groups were activated with glutaraldehyde to produce GA-N-CSMG, and invertase covalently attached by the aldehyde.Invertase was used as a model enzyme to measure the immobilizing character of the GA-N-CSMG material. Using an optimized immobilization protocol, a very high loading of 723 mg invertase per gram GA-N-CSMG is obtained; 3–200-fold higher than values published in literature. The reproducible, immobilized activity of 246,000 U/g GA-N-CSMG is also greater than any other in literature. Immobilized invertase showed almost 99% retention of free enzyme activity and no loss in catalytic efficiency. The apparent kinetic parameters KM and VM were determined using the Michealis–Menten kinetic model. KM of the free invertase was 1.5 times greater than that of the immobilized invertase—indicating a higher substrate affinity of the immobilized invertase. These findings show considerable promise for this material as an immobilization matrix in industrial processes.

Keywords
Enzyme immobilization; Invertase; Amino-functionalized silica; Mesoporous
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Chemically surface modified gel (CSMG): An excellent enzyme-immobilization matrix for industrial processes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 125, Issue 3, 18 September 2006, Pages 395–407
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Online Support
Any Questions? feel free to contact us