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Cloning, purification and characterization of a functional anthracycline glycosyltransferase

Paper ID Volume ID Publish Year Pages File Format Full-Text
25641 43586 2006 9 PDF Available
Title
Cloning, purification and characterization of a functional anthracycline glycosyltransferase
Abstract

We have cloned the gene that encodes a novel glucosyl transferase (AraGT) involved in rhamnosylation of the polyketide antibiotic Aranciamycin in Streptomyces echinatus. AraGT comprises two domains characteristic of bacterial glycosyltranferases. AraGT was synthesized in E. coli as a decahistidinyl-tagged polypeptide. Purified AraGT is dimeric, displays a Tmapp of 30 °C and can glycosylate the aglycone of an Aranciamycin derivative as shown by liquid chromatography and mass spectrometry. The availability of functional AraGT will allow the generation Aranciamycin-based combinatorial libraries.

Keywords
Aranciamycin; Glycosyl transferase; Antibiotics; Anthracyclin; Streptomyces
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Cloning, purification and characterization of a functional anthracycline glycosyltransferase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 125, Issue 3, 18 September 2006, Pages 425–433
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us