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Diversity of plant cell wall esterases in thermophilic and thermotolerant fungi

Paper ID Volume ID Publish Year Pages File Format Full-Text
25642 43586 2006 12 PDF Available
Title
Diversity of plant cell wall esterases in thermophilic and thermotolerant fungi
Abstract

Fourteen thermophilic and thermotolerant fungal strains isolated from composting soils produced plant cell wall-acting esterases in a medium containing corn cobs and oat spelt xylan. The concentrated and dialyzed protein extracts of these fungi were fractionated using isoelectric-focusing, gels sliced and eluted protein in each slice was assayed for esterase activity against p-nitrophenyl acetate. A total of 84 esterases detected on the basis of pI were found to show distinct preferential substrate specificities towards p-nitrophenyl acetate, p-nitrophenyl ferulate and p-nitrophenyl butyrate, and were putatively classified as acetyl esterases and esterases types I and II. None of the esterases were active against p-nitrophenyl myristate. In addition, these esterases were characterized as acid, neutral or alkaline active.

Keywords
Plant cell wall esterases; Thermotolerant and thermophilic fungi; Substrate specificities; Acetyl esterases; Feruloyl esterases
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Diversity of plant cell wall esterases in thermophilic and thermotolerant fungi
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 125, Issue 3, 18 September 2006, Pages 434–445
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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