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Expression, purification and characterization of human GM-CSF using silkworm pupae (Bombyx mori) as a bioreactor

Paper ID Volume ID Publish Year Pages File Format Full-Text
25657 43588 2006 12 PDF Available
Title
Expression, purification and characterization of human GM-CSF using silkworm pupae (Bombyx mori) as a bioreactor
Abstract

To date, many recombinant proteins have been expressed in Bombyx mori cells or silkworm larvae, apart from in pupae. Silkworm pupae may be more suitable for the expression of heterologous proteins as a bioreactor. If maintained at an appropriate temperature, silkworm pupae could be inoculated with recombinant baculovirus for the expression of a protein of interest. In this study, human granulocyte-macrophage colony-stimulating factor was successfully expressed in silkworm pupae using B. mori nucleopolyhedrovirus, purified and characterized with respect to its physico-chemical properties. The target protein expressed had an apparent molecular mass of 29 kDa and an isoelectric point of 5.1. The protein was purified using three chromatographic steps with a final recovery of 10.3%. Finally, approximately 3.5 mg of the protein was obtained with a biological activity of up to 8.4 × 106 cfu mg−1. The results of this study suggest that silkworm pupae represent a convenient and low-cost bioreactor for the expression of heterologous proteins.

Keywords
Human granulocyte-macrophage colony-stimulating factor; Bombyx mori nucleopolyhedrovirus; Silkworm pupae; Expression; Purification; Bioreactor
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Expression, purification and characterization of human GM-CSF using silkworm pupae (Bombyx mori) as a bioreactor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 123, Issue 2, 17 May 2006, Pages 236–247
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us