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Photoreactive cellulose membrane—A novel matrix for covalent immobilization of biomolecules

Paper ID Volume ID Publish Year Pages File Format Full-Text
25670 43589 2006 10 PDF Available
Title
Photoreactive cellulose membrane—A novel matrix for covalent immobilization of biomolecules
Abstract

We report a simple and mild procedure for the preparation of a photoreactive cellulose membrane capable of forming a covalent bond with a biomolecule in presence of 365 nm UV light. Photoreactive cellulose membrane was prepared by the reaction of fluoro group of 1-fluoro-2-nitro-4-azidobenzene (FNAB) and hydroxyl group of the cellulose in an alkaline medium. X-ray photoelectron spectroscopy (XPS) of the photoreactive cellulose confirmed the incorporation of FNAB moiety. Azido group of the photoreactive membrane on exposure to UV light transforms into highly reactive nitrene which binds with a protein. The efficacy of the activated membrane was checked by immobilizing glucose oxidase (GOD) onto it in presence of light. Immobilized GOD was found to have improved thermal, pH and storage stability. Photoreactive cellulose membrane was successfully used in enzyme-linked immunosorbent assay (ELISA) technique. The antibody immobilized onto such support by UV irradiation in 30 min showed similar ELISA value than the antibody immobilized onto a polystyrene ELISA plate in 12 h incubation at 4 °C by conventional method.

Keywords
ELISA, enzyme-linked immunosorbent assay; FNAB, 1-fluoro-2-nitro-4-azidobenzene; GOD, glucose oxidase; XPS, X-ray photoelectron spectroscopyCellulose membrane; Photoimmobilization; ELISA; Enzyme; X-ray photoelectron spectroscopy; 1-Fluoro-2-nitro-4-azidob
First Page Preview
Photoreactive cellulose membrane—A novel matrix for covalent immobilization of biomolecules
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 126, Issue 2, 1 November 2006, Pages 220–229
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering