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Transduction of NeuroD2 protein induced neural cell differentiation

Paper ID Volume ID Publish Year Pages File Format Full-Text
25671 43589 2006 7 PDF Available
Title
Transduction of NeuroD2 protein induced neural cell differentiation
Abstract

NeuroD2, one of the neurospecific basic helix–loop–helix transcription factors, has the ability to induce neural differentiation in undifferentiated cells. In this paper, we show that transduction of NeuroD2 protein induced mouse neuroblastoma cell line N1E-115 into neural differentiation. NeuroD2 has two basic-rich domains, one is nuclear localization signal (NLS) and the other is basic region of basic helix–loop–helix (basic). We constructed some mutants of NeuroD2, ND2Δ100–115 (lack of NLS), ND2Δ123–134 (lack of basic) and ND2Δ100–134 (lack of both NLS and basic) for transduction experiments. Using these proteins, we have shown that NLS region of NeuroD2 plays a role of protein transduction. Continuous addition of NeuroD2 protein resulted in N1E-115 cells adopting neural morphology after 4 days and Tau mRNA expression was increased. These results suggest that neural differentiation can be induced by direct addition of NeuroD2 protein.

Keywords
Neural differentiation; Protein transduction; NeuroD2; N1E-115; Basic helix–loop–helix transcription factor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 126, Issue 2, 1 November 2006, Pages 230–236
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us