fulltext.study @t Gmail

Somatic embryogenesis and plant regeneration from leaf callus of Ocimum basilicum L.

Paper ID Volume ID Publish Year Pages File Format Full-Text
25675 43589 2006 5 PDF Available
Title
Somatic embryogenesis and plant regeneration from leaf callus of Ocimum basilicum L.
Abstract

A effective protocol for complete plant regeneration via somatic embryogenesis has been developed for Ocimum basilicum L. Callus was initiated from leaf explant of young plant on Murashige and Skoog's medium (MS) (1962) supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) 1.0 mg l−1, 3% sucrose and 0.9% agar. The calli showed differentiation of globular structure embryos when transferred to MS medium containing 2,4-D 0.5 mg l−1 and BAP 1.0 mg l−1. The maximum globular structure embryos were further enlarged and produced somatic embryos in MS basal medium supplemented with BAP 1.0 mg l−1 + NAA 1.0 mg l−1 + KN 0.5 mg l−1. Continued formation of globular embryo and germination of embryos occurred in this medium. Complete plantlets were transferred onto specially made plastic cup containing soilrite followed by their transfer to the garden soil. Survival rate of the plantlets under ex vitro condition was 80%.

Keywords
Ocimum basilicum; Callus induction; Somatic embryogenesis; Plant regeneration; Leaf explant
First Page Preview
Somatic embryogenesis and plant regeneration from leaf callus of Ocimum basilicum L.
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 126, Issue 2, 1 November 2006, Pages 260–264
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering