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Antibiosis by Bacillus amyloliquefaciens ribonuclease barnase expressed in Escherichia coli against symbiotic and endophytic nitrogen-fixing bacteria

Paper ID Volume ID Publish Year Pages File Format Full-Text
25681 43590 2006 4 PDF Available
Title
Antibiosis by Bacillus amyloliquefaciens ribonuclease barnase expressed in Escherichia coli against symbiotic and endophytic nitrogen-fixing bacteria
Abstract

A modified antibiosis assay was used to evaluate growth inhibition of symbiotic and endophytic bacteria by E. coli strains producing Bacillus amyloliquefaciens ribonuclease, barnase. Inhibition zones were only observed when the assays were performed in minimal medium agar. However, bacterial growth inhibition was not detected when using rich medium or susceptible strains expressing the ribonuclease inhibitor protein, barstar. Our results suggest that barnase may act as a broad range bacteriocin. The ecological significance of these results is discussed.

Keywords
Barnase; Antibiosis; Symbiotic and endophytic bacteria
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Antibiosis by Bacillus amyloliquefaciens ribonuclease barnase expressed in Escherichia coli against symbiotic and endophytic nitrogen-fixing bacteria
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 126, Issue 3, 10 November 2006, Pages 291–294
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
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