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Direct immobilization of tyrosinase enzyme from natural mushrooms (Agaricus bisporus) on d-sorbitol cinnamic ester

Paper ID Volume ID Publish Year Pages File Format Full-Text
25682 43590 2006 9 PDF Available
Title
Direct immobilization of tyrosinase enzyme from natural mushrooms (Agaricus bisporus) on d-sorbitol cinnamic ester
Abstract

Mushroom tyrosinase was immobilized from an extract onto the totally cinnamoylated derivative of d-sorbitol by direct adsorption as a result of the intense hydrophobic interactions that took place. The immobilization pH value and mass of lyophilized mushrooms were important parameters that affected the immobilization efficiency, while the immobilization time and immobilization support concentration were not important in this respect. The extracted/immobilized enzyme could best be measured above pH 3.5 and the optimum measuring temperature was 55 °C. The apparent Michaelis constant using 4-tert-butylcatechol as substrate was 0.38 ± 0.02 mM, which was lower than for the soluble enzyme from Sigma (1.41 ± 0.20 mM). Immobilization stabilized the extracted enzyme against thermal inactivation and made it less susceptible to activity loss during storage. The operational stability was higher than in the case of the tyrosinase supplied by Sigma and immobilized on the same support. The results show that the use of p-nitrophenol as enzyme-inhibiting substrate during enzyme extraction and immobilization made the use of ascorbic acid unnecessary and is a suitable method for extracting and immobilizing the tyrosinase enzyme, providing good enzymatic activity and stability.

Keywords
Mushroom tyrosinase; Immobilization; Cinnamic carbohydrate esters; Tyrosinase extraction; Enzyme kinetics; Tyrosinase stability
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Direct immobilization of tyrosinase enzyme from natural mushrooms (Agaricus bisporus) on d-sorbitol cinnamic ester
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 126, Issue 3, 10 November 2006, Pages 295–303
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us