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Subunit composition of a large xylanolytic complex (xylanosome) from Streptomyces olivaceoviridis E-86

Paper ID Volume ID Publish Year Pages File Format Full-Text
25683 43590 2006 9 PDF Available
Title
Subunit composition of a large xylanolytic complex (xylanosome) from Streptomyces olivaceoviridis E-86
Abstract

A xylanolytic complex (xylanosome) was isolated from Streptomyces olivaceoviridis E-86 grown on corncob xylan. The isolated xylanosome exhibited a high molecular mass of approximately 3.8 × 107 Da (weight average) using size exclusion chromatography/multi-angle laser light scattering (SEC/MALLS), and was composed of at least 8 subunits with a mass range from 12 to 60 kDa. When a SDS-polyacrylamide gel zymogram was examined, the subunits of 47, 35, 32, and 23 kDa were found to have xylanase activity, while the 30-kDa subunit had CMCase activity. According to N-terminal sequence analyses, the 47- and 23-kDa subunits were found to be identical to the two reported xylanases, namely FXYN and GXYN, of S. olivaceoviridis E-86. Both the 35- and 32-kDa subunits were found to be truncated forms of the intact FXYN xylanase that possibly resulted from the degradation by proteases. The 15-kDa subunit consisted solely the xylan-binding domain of the FXYN xylanase. The purified xylanosome appeared to bind partially to xylan and poorly to Avicel.

Keywords
Size exclusion chromatography/multi-angle laser light scattering (SEC/MALLS); Subunit composition; Streptomyces olivaceoviridis; Xylanosome
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Subunit composition of a large xylanolytic complex (xylanosome) from Streptomyces olivaceoviridis E-86
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 126, Issue 3, 10 November 2006, Pages 304–312
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Price was $35.95
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