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Characterization of Fe-hydrogenase genes diversity and hydrogen-producing population in an acidophilic sludge

Paper ID Volume ID Publish Year Pages File Format Full-Text
25688 43590 2006 8 PDF Available
Title
Characterization of Fe-hydrogenase genes diversity and hydrogen-producing population in an acidophilic sludge
Abstract

According to the DNA sequences of six Fe-hydrogenase genes (FHG) of Clostridium species retrieved from the GenBank, a set of primers specific for Fe-hydrogenase genes were identified from their common conserved regions. The length of DNA fragments amplified using these two primers averaged 313 bps. This primer set was then used to investigate the FHG diversity in an acidophilic rice-degrading sludge by methods based on polymerase chain reaction (PCR). Eight new Fe-hydrogenase gene fragments were identified from the sludge, as a result. Similarity based on amino acids among the 14 hydrogenase genes (8 newly found plus 6 known ones) was 39–97%, which is comparable to the similarity of 41–82% among the 6 known hydrogenase genes alone. The low similarity indicates a great diversity on Fe-hydrogenase among the Clostridium species. The primer set was then used to monitor the change of hydrogen-producing microbial population in a batch reaction using the technique of quantitative real-time polymerase chain reaction (qRT-PCR) with SYBR Green I as the fluorescent reagent. Results showed that the hydrogen producers had an average generation time of 4.2 h, and a production rate of 7.0 × 1016 H2-molecule cell−1 h−1.

Keywords
Acidophilic; Fe-hydrogenase gene; Hydrogen production; Primer; Quantitative real-time polymerase chain reaction
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Characterization of Fe-hydrogenase genes diversity and hydrogen-producing population in an acidophilic sludge
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 126, Issue 3, 10 November 2006, Pages 357–364
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us