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Force response and actin remodeling (agglomeration) in fibroblasts due to lateral indentation

Paper ID Volume ID Publish Year Pages File Format Full-Text
2569 116 2007 11 PDF Available
Title
Force response and actin remodeling (agglomeration) in fibroblasts due to lateral indentation
Abstract

We report the loading and unloading force response of single living adherent fibroblasts due to large lateral indentation obtained by a two-component microelectromechanical systems force sensor. Strong hysteretic force response is observed for all the tested cells. For the loading process, the force response is linear (often with small initial non-linearity) to a deformation scale comparable to the undeformed cell size, followed by plastic yielding. In situ visualization of actin fibers by tagging with green fluorescent protein indicates that during the indentation process, actin network possibly decomposes irreversibly at discrete locations where well-defined circular actin agglomerates appear all over the cell, which explains the irreversibility of the force response. Similar agglomeration is observed when the cell is compressed laterally by a micro plate. The distribution pattern of the agglomerates strongly correlates with the arrangement of the actin fibers of the pre-indented cell. The size of the agglomerates increases with time as tα, initially with α = 2–3 followed by α = 0.5–1. The higher growth rate suggests influx of actin into the agglomerates. The slower rate suggests a diffusive spreading, but the diffusion constant is two orders of magnitude lower than that of an actin monomer through the cytoplasm. Actin agglomeration has previously been observed due to biochemical treatment, gamma-radiation, and ischemic injury, and has been identified as a precursor to cell death. We believe this is the first evidence of actin agglomeration due to mechanical indentation/compression. The study demonstrates that living cells may initiate similar functionalities in response to dissimilar mechanical and biochemical stimuli.

Keywords
Cell mechanics; Large deformation; Microelectromechanical systems (MEMS); Green fluorescent protein (GFP); Actin fibers
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Force response and actin remodeling (agglomeration) in fibroblasts due to lateral indentation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 3, Issue 1, January 2007, Pages 77–87
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us