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Role of proteins in resistance mechanism of Pseudomonas fluorescens against heavy metal induced stress with proteomics approach

Paper ID Volume ID Publish Year Pages File Format Full-Text
25690 43590 2006 9 PDF Available
Title
Role of proteins in resistance mechanism of Pseudomonas fluorescens against heavy metal induced stress with proteomics approach
Abstract

The genus Pseudomonas is a group of gram-negative, motile, rod-shaped bacteria known for their metabolic versatility. One of the species is Pseudomonas fluorescens, which has an efficient system for detoxification of industrial waste. Other aspects include, catabolic versatility, excellent root colonizing abilities and capacity to produce a wide range of antifungal metabolites. They are also known for their resistance and survival in the presence of several organic and inorganic pollutants. P. fluorescens has also been isolated from metal polluted water and soils but the elucidation of proteins responsible for its survival is still not clear. The aim of the study was to elucidate the differential protein expression of this bacterium when exposed to heavy metal stress, using two-dimensional electrophoresis. The proteins spo VG and enolase showed upregulation during the bacterial exposure to lead and copper. Hypothetical protein showed downregulation when bacterium was exposed to cobalt. Some proteins like xylosyltransferase, ORF 18 phage phi KZ, OMP H1 and translational elongation factor EF-Tu appeared only during their exposure to cobalt. These were absent in the control condition. Analysis of the differentially expressed proteins as well as the newly synthesized proteins along with the results obtained growth and enzyme activity indicate the involvement of all these factors in the survival of this organism in the presence of heavy metals.

Keywords
mM, millimolar; min, minute; h, hours; μl, microlitres; MALDI-TOF, MS-matrix assisted laser desorption/ionization-time of flight mass spectrometry; ROS, reactive oxygen speciesEffluents; Heavy metals; Mass spectroscopy; Oxidative stress; Pseudomonas fluor
First Page Preview
Role of proteins in resistance mechanism of Pseudomonas fluorescens against heavy metal induced stress with proteomics approach
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 126, Issue 3, 10 November 2006, Pages 374–382
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering