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Specific activation of the human HSP70 promoter by copper sulfate in mosaic transgenic zebrafish

Paper ID Volume ID Publish Year Pages File Format Full-Text
25693 43590 2006 8 PDF Available
Title
Specific activation of the human HSP70 promoter by copper sulfate in mosaic transgenic zebrafish
Abstract

Heat shock proteins (HSPs) play a central role in cell protection and repair upon stresses, such as that caused by heat and heavy metals. Copper sulfate inducibility of a pHhsp70 construct expressing the enhanced green fluorescent protein (EGFP) gene under the control of the exogenous human hsp70 promoter was tested in transfected CHSE 214 cells and transgenic zebrafish (Danio rerio). We developed a transient expression system, using mosaically transgenic zebrafish, which allows rapid analysis of transgenic expression. Transfected CHSE 214 cells which had been exposed to 250 nM and 2.5 μM copper sulfate for up to 24 h showed increased EGFP expression in a dose-dependent manner. The 1.5 μM copper sulfate caused stronger EGFP fluorescence than the 1.0 μM copper sulfate in transgenic zebrafish. Most of the expression was spotty and was detected in the gills, dorsal and ventral retina, myotubes of the trunk, and skin epithelium. Transgenic zebrafish exposed to copper sulfate exhibited gross dysmorphogenesis, edema and trunk abnormalities, such as spinal lordosis, in vertebral development 5 days after fertilization. This transgenic zebrafish system was sensitive enough to detect copper sulfate at doses below the median lethal concentration (the LC50 was calculated to be 1.2 μM (95% confidence interval of 0.6–1.9 μM)). These results indicate that zebrafish could be useful transgenic biosensor systems for the detection of xenobiotic toxicants in the environment.

Keywords
Copper sulfate; Green fluorescent protein; Human hsp70 promoter; Transgenic zebrafish
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Specific activation of the human HSP70 promoter by copper sulfate in mosaic transgenic zebrafish
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 126, Issue 3, 10 November 2006, Pages 406–413
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us