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Application of “oxygen uptake rate-amino acids” associated mode in controlled-fed perfusion culture

Paper ID Volume ID Publish Year Pages File Format Full-Text
25698 43591 2006 9 PDF Available
Title
Application of “oxygen uptake rate-amino acids” associated mode in controlled-fed perfusion culture
Abstract

Controlled-fed perfusion, a new operation mode, which combines the advantages of fed-batch and perfusion, has been reported to enhance monoclonal antibody productivity. The aim of the present study was to further enrich this mode by an “oxygen uptake rate-amino acids (OUR-AA)” strategy in which the feeding of amino acids was controlled according to the variation of OUR during perfusion. And the effects of this strategy on bioreactor productivity and product quality were evaluated. Experimental results indicated that by using this “OUR-AA” approach in controlled-fed perfusion mode a high viable cell density of more than 1.9 × 107 cells/ml was achieved and the productivity of mAb reached 325 mg/l/d, which was significantly increased by nearly twofold over those of the perfusion and fed-batch process. The residual concentrations of selected amino acids were controlled at a relative steady level by OUR during the culture. The immunoreactivity and the purity of the antibody were well preserved as the culture process was evolving from flask to the controlled-fed perfusion mode. The primary application of “OUR-AA” approach in controlled-fed perfusion mode may present a novel control strategy to enhance the culture performance and to display the potential of this approach in automatic control field.

Keywords
Controlled-fed perfusion; Oxygen uptake rate; Amino acids; Cell specific perfusion rate; Bioreactor; Monoclonal antibody
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Application of “oxygen uptake rate-amino acids” associated mode in controlled-fed perfusion culture
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 122, Issue 4, 20 April 2006, Pages 422–430
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us