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Accumulation of hEGF and hEGF–fusion proteins in chloroplast-transformed tobacco plants is higher in the dark than in the light

Paper ID Volume ID Publish Year Pages File Format Full-Text
25741 43596 2006 14 PDF Available
Title
Accumulation of hEGF and hEGF–fusion proteins in chloroplast-transformed tobacco plants is higher in the dark than in the light
Abstract

Chloroplast transformation has many potential advantages for the production of recombinant proteins in plants. However, it has been reported that heterologous protein accumulation in chloroplasts could be hindered by post-transcriptional mechanisms not yet characterized. Here, we describe the development and characterization of transplastomic tobacco plants transformed with four different transformation vectors for the expression of human epidermal growth factor (hEGF). We showed that, although the corresponding transcript was present in all of the analyzed plants, hEGF could only be detected when fused to the first 186 amino acids of bacterial β-glucuronidase (GUS). In addition, we observed that the expression levels of recombinant protein increased when plants were placed in the dark or when leaves were incubated in the presence of electron transport inhibitors, such as methyl viologen (MV) and 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU). These results suggest that the mechanism responsible for hEGF instability in chloroplasts is regulated by light.

Keywords
hEGF; Chloroplast transformation; Transplastomic tobacco; Molecular farming; Fusion protein
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Accumulation of hEGF and hEGF–fusion proteins in chloroplast-transformed tobacco plants is higher in the dark than in the light
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 125, Issue 2, 1 September 2006, Pages 159–172
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us