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Efficient generation of insect-based cell-free translation extracts active in glycosylation and signal sequence processing

Paper ID Volume ID Publish Year Pages File Format Full-Text
25744 43596 2006 4 PDF Available
Title
Efficient generation of insect-based cell-free translation extracts active in glycosylation and signal sequence processing
Abstract

A novel method for generation of insect-based cell-free translation extracts is presented. The protocol can be completed in less than an hour, and the resulting extracts are extremely proficient in N-linked glycosylation and signal sequence processing. No specialized equipment other than that usually present in an ordinary biochemistry laboratory is required. The novel approach dramatically reduces cost and time while rendering enhanced lysates compared to previously published strategies.

Keywords
In vitro protein expression; N-linked glycosylation; Signal sequence processing
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Efficient generation of insect-based cell-free translation extracts active in glycosylation and signal sequence processing
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 125, Issue 2, 1 September 2006, Pages 194–197
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us