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Inhibition of cellulase, xylanase and β-glucosidase activities by softwood lignin preparations

Paper ID Volume ID Publish Year Pages File Format Full-Text
25745 43596 2006 12 PDF Available
Title
Inhibition of cellulase, xylanase and β-glucosidase activities by softwood lignin preparations
Abstract

The conversion of lignocellulosic biomass to fuel ethanol typically involves a disruptive pretreatment process followed by enzyme-catalyzed hydrolysis of the cellulose and hemicellulose components to fermentable sugars. Attempts to improve process economics include protein engineering of cellulases, xylanases and related hydrolases to improve their specific activity or stability. However, it is recognized that enzyme performance is reduced during lignocellulose hydrolysis by interaction with lignin or lignin–carbohydrate complex (LCC), so the selection or engineering of enzymes with reduced lignin interaction offers an alternative means of enzyme improvement. This study examines the inhibition of seven cellulase preparations, three xylanase preparations and a β-glucosidase preparation by two purified, particulate lignin preparations derived from softwood using an organosolv pretreatment process followed by enzymatic hydrolysis. The two lignin preparations had similar particle sizes and surface areas but differed significantly in other physical properties and in their chemical compositions determined by a 2D correlation HSQC NMR technique and quantitative 13C NMR spectroscopy. The various cellulases differed by up to 3.5-fold in their inhibition by lignin, while the xylanases showed less variability (≤1.7-fold). Of all the enzymes tested, β-glucosidase was least affected by lignin.

Keywords
Lignin; Softwood; Inhibition; Cellulase; Xylanase; β-Glucosidase; NMR spectroscopy
First Page Preview
Inhibition of cellulase, xylanase and β-glucosidase activities by softwood lignin preparations
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 125, Issue 2, 1 September 2006, Pages 198–209
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering