fulltext.study @t Gmail

A novel transfection method for mammalian cells using gas plasma

Paper ID Volume ID Publish Year Pages File Format Full-Text
25758 43597 2006 10 PDF Available
Title
A novel transfection method for mammalian cells using gas plasma
Abstract

Introduction of foreign genes into target cells is a crucial step for achievement of gene therapy. We have recently developed a novel transfection system for eukaryotic cells, namely the electric pulse-activated gas plasma generator. To measure the transfection efficiency and mortality by flow-cytometry, we employed enhanced green fluorescent protein and propidium iodide staining, respectively. One day after the 1–3 s plasma exposures with DNA concentration at 0.5 μg/μl, favorable transfection efficiencies (17.8–21.6%) and mortalities (0.65–2.86%) were obtained for HeLa-S3, HT-1080 and MCF-7 cells. The recipient cells became transiently permeable for plasmid DNA during the plasma exposure, suggesting that plasma-mediated transfection may involve similar mechanisms that accounts for electroporation. The relatively low mortality rates are encouraging in our attempt to apply this system to the various cell lines including the primary cell cultures.

Keywords
Electric pulse; Gas plasma; Transfection efficiency; Mortality; Enhanced green fluorescent protein (EGFP); Electroporation
First Page Preview
A novel transfection method for mammalian cells using gas plasma
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 121, Issue 3, 10 February 2006, Pages 299–308
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us