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ITS primers for the identification of marketable boletes

Paper ID Volume ID Publish Year Pages File Format Full-Text
25760 43597 2006 12 PDF Available
Title
ITS primers for the identification of marketable boletes
Abstract

Boletus species belonging to the section Boletus are the most frequently eaten fungi among those harvested in natural conditions in Europe. This section groups 10 taxa which are hardly distinguishable on the basis of their morphology. Some of them have been shown to induce allergic IgE-mediated symptoms either through inhalation, ingestion or contact.Since questions relating to the presence of allergens in any of the species most in demand (B. edulis, B. aereus, B. pinophilus, B. aestivalis, all classified as B. edulis s.l.) remain open, together with the absence of tools which distinguish the species, we sequenced the ITS region of 28 Boletus samples and then we designed specific primers. These allowed the effective separation of the taxa. In addition, the phylogenetic tree obtained from the sequences alignment revealed that B. violaceofuscus, a spectacular Chinese fungus considered belonging to the section Boletus and often sold intermixed with B. edulis s.l. specimens, clusters outside the section Boletus.

Keywords
Boletus edulis s.l.; ITS primers; Phylogenetic analysis
First Page Preview
ITS primers for the identification of marketable boletes
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 121, Issue 3, 10 February 2006, Pages 318–329
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering