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Two-dimensional fluorescence spectroscopy: A novel approach for controlling fed-batch cultivations

Paper ID Volume ID Publish Year Pages File Format Full-Text
25770 43597 2006 8 PDF Available
Title
Two-dimensional fluorescence spectroscopy: A novel approach for controlling fed-batch cultivations
Abstract

In industrial fed-batch cultivations it is often necessary to control substrate concentrations at a low level to prevent the production of overflow metabolites and thus optimize the biomass yield. A new method for on-line monitoring and fed-batch control based on fluorescence measurements has been developed. Via instantaneous in situ measurements and multivariate data analysis a chemometric model has been established, which enables the rapid detection of ethanol production at aerobic Saccharomyces cerevisiae fed-batch cultivations. The glucose feed rate is controlled by predicting the metabolic state directly from the fluorescence intensities. Thus, ethanol production could be avoided completely while increasing the biomass yield accordingly. The robust instrumentation is suitable for industrial applications.

Keywords
Fed-batch; Fluorescence; On-line monitoring; Ethanol inhibition; S. cerevisiae
First Page Preview
Two-dimensional fluorescence spectroscopy: A novel approach for controlling fed-batch cultivations
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 121, Issue 3, 10 February 2006, Pages 410–417
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering