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Analysis of polarity in the expression from a multifactorial bidirectional promoter designed for high-level expression of transgenes in plants

Paper ID Volume ID Publish Year Pages File Format Full-Text
25773 43599 2006 12 PDF Available
Title
Analysis of polarity in the expression from a multifactorial bidirectional promoter designed for high-level expression of transgenes in plants
Abstract

A synthetic bidirectional expression module was constructed by placing a computationally designed minimal promoter sequence on the 5′ and 3′ sides of a transcription activation module. The activation of transcription from the unidirectional and bidirectional promoters constructed from the same sequence elements was evaluated by using the reporter genes gusA and gfp. The analysis based on transient and stable transformation of tobacco showed that the artificially designed multifactorial activation module activated transcription simultaneously to comparable levels in both the directions. The transcription activation module responded to elicitors like salicylic acid, NaCl and IAA in the forward as well as reverse directions. The concentration of the elicitor required for highest gene activation was similar for the two directions in case of the three activators. The kinetics of time of induction was similar in the two directions for salicylic acid and NaCl. In the case of IAA, the transcription activation was faster in the reverse direction. The results show that constitutive and chemically inducible bidirectional promoters can be deployed for predictable simultaneous regulation of two genes for genetic engineering in plants.

Keywords
Bidirectional promoter; Gene expression; Plant vectors; Polar promoter; Promoter
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Analysis of polarity in the expression from a multifactorial bidirectional promoter designed for high-level expression of transgenes in plants
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 123, Issue 1, 3 May 2006, Pages 1–12
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us