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A nanocompartment system (Synthosome) designed for biotechnological applications

Paper ID Volume ID Publish Year Pages File Format Full-Text
25778 43599 2006 10 PDF Available
Title
A nanocompartment system (Synthosome) designed for biotechnological applications
Abstract

A nanocompartment system based on two deletion mutants of the large channel protein FhuA (FhuA Δ1–129; FhuA Δ1–160) and an ABA triblock copolymer (PMOXA–PDMS–PMOXA) has been developed for putative biotechnological applications. FhuA is ideally suited for applications in biotechnology due to its monomeric structure, large pore diameter (39–46 Å elliptical cross-section) that ensures rapid compound flux, and solved crystallographic structure. Two areas of application were targeted as proof of principle: (A) selective product recovery in nanocompartments and (B) enzymatic conversion in nanocompartments. Selective recovery of negatively charged compounds has been achieved on the example of sulforhodamine B by using positively charged polylysine molecules as trap inside the nanocompartment. Conversion in nanocompartments has been achieved by 3,3′,5,5′-tetramethylbenzidine oxidation employing horseradish peroxidase (HRP).

Keywords
Nanocompartment; Block copolymer; Vesicle; Transmembrane channel; FhuA
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A nanocompartment system (Synthosome) designed for biotechnological applications
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 123, Issue 1, 3 May 2006, Pages 50–59
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us