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Optimization of culture conditions and medium composition for the production of micrococcin GO5 by Micrococcus sp. GO5

Paper ID Volume ID Publish Year Pages File Format Full-Text
25792 43601 2006 8 PDF Available
Title
Optimization of culture conditions and medium composition for the production of micrococcin GO5 by Micrococcus sp. GO5
Abstract

To enhance the production of micrococcin GO5, a bacteriocin produced by Micrococcus sp. GO5, cultivation conditions and medium composition were optimized. The optimal initial pH and temperature for bacteriocin production were 7.0–9.0 and 37 °C, respectively. Micrococcus sp. GO5 displayed the highest micrococcin GO5 activity when grown in modified MRS medium that contained lactose or sucrose, rather than glucose, as a carbon source. The maximum bacteriocin activity was obtained in modified MRS medium containing 0.5% tryptone and 1.0% yeast extract as nitrogen sources instead of the other nitrogen sources present in MRS medium. Bacteriocin production was greatly affected by the concentration of K2HPO4; strain GO5 produced eight-fold more bacteriocin in medium containing 2.0–2.5% K2HPO4 than in medium containing 0.2% K2HPO4. The optimal concentration of MgSO4·7H2O for bacteriocin production was 0.5%. The production of micrococcin GO5 was increased 32-fold in shake flask culture and 16-fold in a bioreactor using the optimized medium (TY medium), compared with culturing in MRS medium.

Keywords
Bacteriocin; Micrococcin; Micrococcus sp.; Kimchi; Production
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Optimization of culture conditions and medium composition for the production of micrococcin GO5 by Micrococcus sp. GO5
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 121, Issue 1, 2 January 2006, Pages 54–61
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us