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Characterization of heterologous hemoglobin and flavohemoglobin promoter regulation in Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
25798 43602 2006 15 PDF Available
Title
Characterization of heterologous hemoglobin and flavohemoglobin promoter regulation in Escherichia coli
Abstract

Bacterial hemoglobins and flavohemoglobins have been used to improve cell growth and productivity in biotechnological applications. The expression of globin genes can be induced by reducing the oxygen supply or applying external stressors, which provide a simple and inexpensive mechanism for induction of heterologous protein production. It is in the interest of the biotechnological industry to seek new promoters, which are non-patented, cheap and simple to induce. Therefore, new globin gene promoters have been isolated from Campylobacter jejuni, Bacillus subtilis, Deinococcus radiodurans, Streptomyces coelicolor, and Salmonella typhi. The goal was to obtain insights about the regulation mechanisms of these promoters in Escherichia coli using in silico and experimental methods. The recognition of these promoters by the E. coli transcriptional machinery was first analyzed by computational methods. Computer analysis revealed that all the promoters, except the promoter of S. coelicolor, should be functional in E. coli and most of them also contain putative binding sites for ArcA, CRP, and FNR global regulators. Furthermore, the expression profiles of the promoters fused to the chloramphenicol acetyl transferase gene were analyzed under various conditions using E. coli mutants devoid of regulatory molecules. In vivo regulation studies of globin promoters mainly verified the in silico predictions.

Keywords
ArcAB, aerobic respiration control; ArcA, the response regulator component; ArcB, response sensor kinase of ArcA; CAT, chloramphenicol acetyl transferase; cat, the chloramphenicol acetyl transferase gene; chb, hemoglobin gene of Campylobacter jejuni; cya,
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Characterization of heterologous hemoglobin and flavohemoglobin promoter regulation in Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 122, Issue 2, 23 March 2006, Pages 161–175
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us