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Stress-related differential expression of multiple β-carotene ketolase genes in the unicellular green alga Haematococcus pluvialis

Paper ID Volume ID Publish Year Pages File Format Full-Text
25799 43602 2006 10 PDF Available
Title
Stress-related differential expression of multiple β-carotene ketolase genes in the unicellular green alga Haematococcus pluvialis
Abstract

The unicellular green alga Haematococcus pluvialis is used as a biological production system for astaxanthin. It accumulates large amounts of this commercially interesting ketocarotenoid under a variety of environmental stresses. Here we report the identification and expression of three different β-carotene ketolase genes (bkt) that are involved in the biosynthesis of astaxanthin in a single strain of the alga. Bkt1 and bkt2 proved to be the crtO and bkt previously isolated from two different strains of H. pluvialis. Bkt3 is a novel third gene, which shared 95% identical nucleotide sequence with bkt2. Nitrogen deficiency alone could not induce the alga cells to produce astaxanthin in 3 days even though it enhances the expression of the bkt genes to three times of that in normal growing cells within 16 h. High light irradiation (125 μmol m−2 s−1) or 45 mM sodium acetate greatly increased the expression of bkt genes to 18 or 52 times of that in normal growing cells, resulting in an accumulation of substantial astaxanthin (about 6 mg g−1 dry biomass) in 3 days. It is suggested that the existence of the multiple bkt genes and their strong up-regulation by different stress conditions is one of the reasons that H. pluvialis accumulates large amounts of astaxanthin in an instant response to stress environments.

Keywords
Astaxanthin; β-Carotene ketolase; Haematococcus pluvialis; Physiological conditions; Real-time PCR
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Stress-related differential expression of multiple β-carotene ketolase genes in the unicellular green alga Haematococcus pluvialis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 122, Issue 2, 23 March 2006, Pages 176–185
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us