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Hydrolyses and transglycosylations performed by purified α-d-glucosidase of the marine mollusc Aplysia fasciata

Paper ID Volume ID Publish Year Pages File Format Full-Text
25808 43602 2006 11 PDF Available
Title
Hydrolyses and transglycosylations performed by purified α-d-glucosidase of the marine mollusc Aplysia fasciata
Abstract

The purification and characterisation of the α-glucosidase from the marine mollusc Aplysia fasciata are reported. Overall substrate specificity of the pure enzyme for both hydrolytic and transglycosylation reactions was studied. Remarkable characteristics of this enzyme are indicated by the results of the interesting survey of transglycosylation reactions reported: pyridoxine glucosylation, synthesis of chromophoric (pNP) di- and trisaccharides, glucosylation of cellobiose and sucrose. For these last two acceptors both the yields of reactions and the concentrations of products are comparable to those obtained using glycosyl transferases; in addition, synthesis of pyridoxine and chromophoric glycosides were still possible using a 1:1 ratio maltose:acceptor which is a very interesting characteristic from a synthetic point of view (effortless purification, productivity of each reaction batch, etc.).

Keywords
α-Glucosidase; Transglycosylation; Oligosaccharide synthesis; Marine organism; Aplysia fasciata; Biocatalysis
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Hydrolyses and transglycosylations performed by purified α-d-glucosidase of the marine mollusc Aplysia fasciata
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 122, Issue 2, 23 March 2006, Pages 274–284
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us