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The full-length clone of cucumber green mottle mosaic virus and its application as an expression system for Hepatitis B surface antigen

Paper ID Volume ID Publish Year Pages File Format Full-Text
25814 43603 2006 11 PDF Available
Title
The full-length clone of cucumber green mottle mosaic virus and its application as an expression system for Hepatitis B surface antigen
Abstract

A cucumber green mosaic mottle virus (CGMMV) full-length clone was developed for the expression of Hepatitis B surface antigen (HBsAg). The expression of the surface displayed HBsAg by the chimeric virus was confirmed through a double antibody sandwich ELISA. Assessment of the coat protein composition of the chimeric virus particles by SDS-PAGE analysis showed that 50% of the coat proteins were fused to the HBsAg. Biological activity of the expressed HBsAg was assessed through the stimulation of in vitro antibody production by cultured peripheral blood mononuclear cells (PBMC). PBMC that were cultured in the presence of the chimeric virus showed up to an approximately three-fold increase in the level of anti HBsAg immunoglobulin thus suggesting the possible use of this new chimeric virus as an effective Hepatitis B vaccine.

Keywords
CGMMV; Infectious transcripts; HBsAg; Tobamovirus; Chimeric virus
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The full-length clone of cucumber green mottle mosaic virus and its application as an expression system for Hepatitis B surface antigen
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 121, Issue 4, 24 February 2006, Pages 471–481
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us