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Identification of secreted proteins of Aspergillus oryzae associated with growth on solid cereal substrates

Paper ID Volume ID Publish Year Pages File Format Full-Text
25815 43603 2006 4 PDF Available
Title
Identification of secreted proteins of Aspergillus oryzae associated with growth on solid cereal substrates
Abstract

Filamentous growth of Aspergillus oryzae on solid cereal substrates involves secretion of substrate converting enzymes and a solid substrate specific polarised hyphal growth phenotype. To identify proteins produced under these specific conditions, the extracts of A. oryzae grown on wheat-based media were analysed using N-terminal sequence analysis. In a submerged wheat-based growth medium of A. oryzae, besides α-amylase, also an arabinosidase and xylanase were abundantly produced. In the extracts of A. oryzae grown on wheat-based solid substrate besides α-amylase and chitinase, two new proteins of 16 and 27 kDa were identified. These hypothetical proteins showed only close homologies to filamentous fungal proteins.

Keywords
Aspergillus oryzae; Filamentous; Cereal substrates
First Page Preview
Identification of secreted proteins of Aspergillus oryzae associated with growth on solid cereal substrates
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 121, Issue 4, 24 February 2006, Pages 482–485
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering