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Cultivation of three-dimensional cartilage-carrier-constructs under reduced oxygen tension

Paper ID Volume ID Publish Year Pages File Format Full-Text
25816 43603 2006 12 PDF Available
Title
Cultivation of three-dimensional cartilage-carrier-constructs under reduced oxygen tension
Abstract

Three-dimensional cartilage-carrier-constructs were produced according to a standard protocol from chondrocytes of an adult mini-pig. Experiments with different oxygen concentrations (21, 10 and 5%, v/v O2) were performed and the constructs were compared qualitatively and quantitatively. The appearance of the cartilage obtained under reduced oxygen tension seemed to be closer to native cartilage with respect to shape of the cells, distribution of the cells within the matrix, smoothness of the surface, etc. The thickness of the cartilage formed by free swelling was always in the same range as for native cartilage (approximately 1 mm). Qualitatively the most stable attachment of the cartilage on top of the carrier was found for 10% O2 (v/v). Especially at 5% O2 (v/v) the attachment between cartilage and carrier was not sufficient. The constructs generated at lower oxygen tensions had a significantly higher amount of glycosaminoglycan per DNA, but still significantly less when compared to native cartilage. Furthermore, the cultivated cartilage contained a large amount of collagen type II. The experiments proved the applied concept for generation of cartilage-carrier-constructs and the usefulness of cultivation under reduced oxygen tension.

Keywords
Cartilage; Chondrocytes; Collagen; Glycosaminoglycan; Oxygen concentration; Tissue engineering
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 121, Issue 4, 24 February 2006, Pages 486–497
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us