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Characterization of d-ribose biosynthesis in Bacillus subtilis JY200 deficient in transketolase gene

Paper ID Volume ID Publish Year Pages File Format Full-Text
25818 43603 2006 9 PDF Available
Title
Characterization of d-ribose biosynthesis in Bacillus subtilis JY200 deficient in transketolase gene
Abstract

d-Ribose is a functional five-carbon sugar, which has been used for the commercial production of riboflavin. Mechanisms of d-ribose biosynthesis from xylose were investigated in the genetically engineered Bacillus subtilis JY200 with a deficiency in transketolase. A transketolase gene (tkt) disruption cassette in plasmid pUNKC was introduced into the chromosomal tkt gene in the wild type B. subtilis 168. Analysis of culture broth by thin layer chromatography confirmed that the disruption of tkt allowed B. subtilis JY200 to produce d-ribose. In a batch culture of B. subtilis JY200, a loss of cell viability was observed after glucose depletion. Fed-batch cultivation by feeding 400 g l−1 glucose solution as a co-substrate was carried out to supply energy to xylose metabolism and to maintain cell viability throughout cultivation. Fed-batch cultivation of B. subtilis JY200 in a complex medium containing 11 g l−1 xylose and 5 g l−1 glucose initially gave the best result of 10.1 g l−1d-ribose concentration, 0.24 g g−1d-ribose yield and 0.29 g l−1 h−1 productivity, corresponding to 40-, 5- and 12-fold increases compared with those in the batch culture. A kinetic study of d-ribose production in fed-batch cultivations of B. subtilis JY200 suggested that xylose uptake might be critical to maximize d-ribose biosynthesis from xylose.

Keywords
d-Ribose; Xylose; Bacillus subtilis; Transketolase; Fed-batch culture; Kinetics
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Characterization of d-ribose biosynthesis in Bacillus subtilis JY200 deficient in transketolase gene
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 121, Issue 4, 24 February 2006, Pages 508–516
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us