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Chemical stabilization of the phycocyanin from cyanobacterium Spirulina platensis

Paper ID Volume ID Publish Year Pages File Format Full-Text
25823 43603 2006 7 PDF Available
Title
Chemical stabilization of the phycocyanin from cyanobacterium Spirulina platensis
Abstract

Phycocyanin (PC) prepared from a cyanobacterium Spirulina platensis by the DEAE-DE52 cellulose column chromatography that was developed by gradient elution of 50–250 mM phosphate buffer (pH 7.0) was stabilized by its subunits cross-linked covalently with formaldehyde. The single blue band that the chemically stabilized PC showed in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that the stabilized PC still maintained its trimeric aggregate form even after its incubation at 60 °C for 3 h and at 100 °C for 10 min in the denatured buffer containing 5% (w/v) SDS. Moreover, the stabilized PC exhibited similar spectroscopic properties of absorption and fluorescence to those of the native PC, and showed adequate energy coupling with R-phycoerythrin (R-PE) after it was conjugated with R-PE via glutaraldehyde.

Keywords
Cyanobacterium; Phycocyanin; Phycoerythrin; Fluorescent Probe; Spirulina platensis
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Chemical stabilization of the phycocyanin from cyanobacterium Spirulina platensis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 121, Issue 4, 24 February 2006, Pages 563–569
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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