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Novel block copolymer (PPDO/PLLA-b-PEG): Enhancement of DNA uptake and cell transfection

Paper ID Volume ID Publish Year Pages File Format Full-Text
2587 117 2006 6 PDF Available
Title
Novel block copolymer (PPDO/PLLA-b-PEG): Enhancement of DNA uptake and cell transfection
Abstract

The cationic lipid mediated uptake of plasmid DNA by cells in monolayer culture was significantly enhanced with an aqueous solution of the block copolymer poly(p-dioxanone-co-l-lactide)-b-poly(ethylene glycol) (PPDO/PLLA-b-PEG). Plasmid uptake studies with DNA encoding the β-galactosidase gene and cytotoxicity evaluations were performed on MCF-7, NIH 3T3 and CT-26 cell lines. Transfection yields and time courses for maximum release of FITC labeled DNA in MCF-7 cells were observed and quantified by β-galactosidase assay and spectrofluorometry, respectively. The reported results suggest that the studied block copolymer might be useful for the enhancement of polycation mediated transfection and could find application in gene therapy.

Keywords
Poly(p-dioxanone-co-l-lactide)-b-poly(ethylene glycol); Biodegradable DNA; Cell transfection; Cell proliferation
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Novel block copolymer (PPDO/PLLA-b-PEG): Enhancement of DNA uptake and cell transfection
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 2, Issue 2, March 2006, Pages 207–212
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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Any Questions? feel free to contact us