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Photochemical behavior of Safranine-Riboflavin complex in the degradation of folic acid

Paper ID Volume ID Publish Year Pages File Format Full-Text
25883 43907 2014 8 PDF Available
Title
Photochemical behavior of Safranine-Riboflavin complex in the degradation of folic acid
Abstract

•The low value of ΔG of complexation between safranine and riboflavin indicates an energetically favorable process.•Photo-induced intermolecular electron transfer was studied within the scope of sensitizer design.•Semioxidized radical of riboflavin and semireduced radical of safranine were generated following safranine sensitization.•Folic acid is degraded by safranine sensitization through a radical pathway.

The photochemistry of safranine complexed with riboflavin and the photoinduced electron transfer from folic acid to the complex were investigated by steady-state and time resolved absorption spectroscopy, and the photoproducts were further analyzed by LC-orbitrap–FT–MS. UV–vis spectra of the complex showed a decrease in the safranine band at 520 nm with increasing concentration of riboflavin, which was attributed to complex formation. A 1:1 (safranine:riboflavin) stoichiometry was determined using Job's method. Following safranine photoexcitation, there is formation of a radical pair in the complex by donation of an electron from ground-state riboflavin to excited-state safranine. The cation radical of riboflavin within this complex reacts with folic acid to yield FA+•, which may accept an electron from the anion radical of safranine, avoiding the photobleaching of the safranine. In contrast, the reaction of the anion radical of non-complexed safranine with folic acid leads to safranine photobleaching.

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Keywords
Dyes; Riboflavin; Safranine; Folic acid; Photochemistry; Electron transfer
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Photochemical behavior of Safranine-Riboflavin complex in the degradation of folic acid
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 293, 1 November 2014, Pages 32–39
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us