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Synthesis and characterization of biologically stable, doped LaF3 nanoparticles co-conjugated to PEG and photosensitizers

Paper ID Volume ID Publish Year Pages File Format Full-Text
25976 43926 2016 9 PDF Available
Title
Synthesis and characterization of biologically stable, doped LaF3 nanoparticles co-conjugated to PEG and photosensitizers
Abstract

•LaF3 nanoparticles doped with terbium and/or cerium are synthesized.•The surface ligand is exchanged to alendronate.•Rose Bengal and chlorin e6 are covalently bound to the nanoparticle surface.•Evidence of cell uptake, scintillation and energy transfer is presented.

There has been significant recent interest in combining radiation therapy and photodynamic therapy for cancer treatment. One proposed method involves conjugating photosensitizers (PS) to scintillating nanoparticles. However, the production of nanoscintillators that are stable in biological media is problematic since many of the commonly used compositions chelate phosphate. In this paper, the problem was solved by first synthesizing LaF3 nanoparticles, followed by surface modification with alendronate, which serves as a stable anchor for conjugation of molecules of interest. The nanoparticles had an average diameter of 4 nm, and were doped with either Ce3+, Tb3+, or both. In every case, a LaF3 shell was added. In subsequent steps, poly(ethylene) glycol (PEG) and photosensitizers (either chlorin e6 or rose bengal) were covalently linked to the alendronate ligand via an amide bond. This new formulation is stable in several solutions and buffers used commonly in the laboratory for cell culture. Moreover, cellular uptake and lack of toxicity up to a concentration of 50 μM were observed in B16 murine melanoma cells. Energy transfer between the doped nanoparticle core and the conjugated photosensitizers was demonstrated using UV excitation, and scintillation was demonstrated using x-rays.

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Keywords
Radiotherapy; Photodynamic therapy; Lanthanum fluoride; Lanthanide; Nanoscintillator; PEG; Rose bengal; Chlorin e6
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Synthesis and characterization of biologically stable, doped LaF3 nanoparticles co-conjugated to PEG and photosensitizers
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 329, 1 October 2016, Pages 26–34
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us