fulltext.study @t Gmail

Morphological and functional studies of rat hepatocytes on a hydrophobic or hydrophilic polydimethylsiloxane surface

Paper ID Volume ID Publish Year Pages File Format Full-Text
2602 118 2009 8 PDF Available
Title
Morphological and functional studies of rat hepatocytes on a hydrophobic or hydrophilic polydimethylsiloxane surface
Abstract

This study describes the morphological and functional behavior of rat hepatocytes on a polydimethylsiloxane (PDMS)-coated surface. Hepatocytes were cultured on hydrophobic or hydrophilic PDMS-coated surfaces in serum-free and serum-containing media. In the serum-free medium, almost all hepatocytes adhered onto the surface irrespective of the wettability, with a cell adhesion ratio of >90% at 24 h. In the serum-containing medium, although they strongly adhered onto the hydrophilic surface (cell adhesion ratio >85%), the ratio on the hydrophobic surface was <15%. Furthermore, hepatocytes in the serum-free medium gradually formed spheroids irrespective of the surface characteristics; however, on the hydrophilic surface in the serum-containing medium, they maintained a monolayer configuration for up to 10 days, and their numbers gradually decreased over time. Expression levels of the functional activities (albumin secretion and ammonia removal) and the cell–cell adhesion molecules (cadherin and connexin-32) were higher in the hepatocytes that formed spheroids compared to those which assumed a monolayer configuration, and these levels were maintained for at least 10 days. These results suggest that the wettability of PDMS and the composition of the culture medium together control the cell adhesion, morphology and expression of functional genes in hepatocytes.

Keywords
Rat hepatocytes; PDMS; Hydrophobic; Hydrophilic; FBS
First Page Preview
Morphological and functional studies of rat hepatocytes on a hydrophobic or hydrophilic polydimethylsiloxane surface
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 5, Issue 2, February 2009, Pages 613–620
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us