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Effect of pore size on ECM secretion and cell growth in gelatin scaffold for articular cartilage tissue engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
2608 118 2009 10 PDF Available
Title
Effect of pore size on ECM secretion and cell growth in gelatin scaffold for articular cartilage tissue engineering
Abstract

A novel method for the preparation of gelatin scaffolds was designed by varying the crosslinking temperature. Four pore size ranges of genipin-crosslinked gelatin scaffolds were made by varying the crosslinking temperature from 10 to 25 °C, with the pore sizes ranging from 50 to 500 μm. The pore size of the scaffold increases as the crosslinking temperature increases. Articular chondrocytes of Wistar rats were in vitro cultured in these scaffolds. DNA assay, glycosaminoglycan (GAG) assay, hematoxylin–eosin staining, Safranin-O staining and reverse transcription-polymerase chain reaction were performed to analyze the effect of the pore size on cell growth and the secretion of extracellular matrix (ECM). As the pores become larger, the rate of cell growth and the amount of GAG secretion increase, and the expressions of all four gene markers for aggrecan, collagen type I, collagen type II and collagen type X increase. The cells in the smaller pores often show a dedifferentiated form. The phenotype of the cells is maintained better in larger pores. Chondrocytes prefer the group of scaffolds with pore size between 250 and 500 μm for better proliferation and ECM production. The size of the space for cell growth is a key factor for cell metabolism.

Keywords
Gelatin scaffold; Genipin crosslinking; Pore size; Extracellular matrix; Articular cartilage
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Effect of pore size on ECM secretion and cell growth in gelatin scaffold for articular cartilage tissue engineering
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 5, Issue 2, February 2009, Pages 670–679
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us