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Selection process generating peptide aptamers and analysis of their binding to the TiO2 surface of a surface acoustic wave sensor

Paper ID Volume ID Publish Year Pages File Format Full-Text
2621 118 2009 7 PDF Available
Title
Selection process generating peptide aptamers and analysis of their binding to the TiO2 surface of a surface acoustic wave sensor
Abstract

The set-up presented in this article is intended for the selection of peptides which serve as specific binders to suitable materials. Additionally, the interaction of such binders with material surfaces can be characterized. Using this approach, a subset of peptides which adhere to the mineral TiO2 was generated by means of a cell surface display library. The peptides are constrained by a thioredoxin scaffold. Selection of proteins was carried out on a silicium wafer sputtered with TiO2 in anatase conformation. To verify binders and to analyze the binding kinetics of the diluted suspension of the purified proteins, the chip-based S-sens® K5 surface acoustic wave sensor system was used. The surface of the sensor chips was also TiO2, resembling the material of the Si wafer selection target retaining the peptides. Several peptides were identified. The respective binding behavior differed. The data derived from real-time interaction analysis were evaluated to select for strong and specific binders. For one of these peptides, the binding kinetics was analyzed. On- and off-rate binding constants were extracted from the fitted curves. With the resulting association rate constant kon and the dissociation constant koff, the affinity of the peptide for the TiO2 surface was calculated, represented by the equilibrium dissociation constant KD = 81 nM.

Keywords
SAW biosensor; TiO2; Bacterial display; Inorganic binding peptide; Peptide aptamers
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Selection process generating peptide aptamers and analysis of their binding to the TiO2 surface of a surface acoustic wave sensor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 5, Issue 2, February 2009, Pages 794–800
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us