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Human osteosarcoma cell adhesion behaviour on hydroxyapatite integrated chitosan–poly(acrylic acid) polyelectrolyte complex

Paper ID Volume ID Publish Year Pages File Format Full-Text
2631 119 2006 7 PDF Available
Title
Human osteosarcoma cell adhesion behaviour on hydroxyapatite integrated chitosan–poly(acrylic acid) polyelectrolyte complex
Abstract

A novel degradable composite system has been prepared by integrating hydroxyapatite, Ca10(PO4)6(OH)2, (HAP) in a polyelectrolyte complex matrix of chitosan (CHI) and poly(acrylic acid) (PAA). The composite was formulated by integrating 80 wt.% HAP in the polyelectrolyte complex matrix of CHI and PAA in the ratio 40/60 (designated as CPH). The composite could be easily fabricated into clinically significant shapes by a simple moulding procedure intended for bone graft applications. The adhesion behaviour of human osteosarcoma (HOS) cells on this degradable composite system was studied by selecting the polyelectrolyte complex, CHI/PAA 40/60 (designated as CP) as control sample. Light microscopic observations show that cells around CPH retained the typical morphology of HOS cells while cells around the polyelectrolyte complex showed a cytotoxic effect. The adhesion behaviour as well as morphological responses of the seeded cells was further investigated by scanning electron microscopy. The scanning electron micrographs of the polyelectrolyte complex, CP, showed the presence of rounded cells with raised nuclear regions, indicating delayed spreading; cells adhered on CPH were flattened with filopodia and showed good attachment and spreading, indicating better adhesion onto the HAP integrated composite. Comparing the MTT assay for quantitative evaluation of cell viability, CPH showed a higher percentage of metabolically active cells compared to CP.

Keywords
Cell adhesion; Chitosan; Hydroxyapatite; Poly(acrylic acid); Polyelectrolyte complex
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Human osteosarcoma cell adhesion behaviour on hydroxyapatite integrated chitosan–poly(acrylic acid) polyelectrolyte complex
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 2, Issue 6, November 2006, Pages 651–657
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us