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Development of a selenide-based fluorescent probe for imaging hypochlorous acid in lysosomes

Paper ID Volume ID Publish Year Pages File Format Full-Text
26386 43950 2015 8 PDF Available
Title
Development of a selenide-based fluorescent probe for imaging hypochlorous acid in lysosomes
Abstract

•A lysosome-targetable fluorescent probe for HOCl was synthesized.•It showed a fast fluorescence response to HOCl in aqueous solution.•The probe displayed a large fluorescence enhancement.•Imaging of exogenous HOCl in lysosomes of living cells was achieved using fluoresce microscope.

The development of fluorescent probes for hypochlorous acid (HOCl) has received intense attention because of the biological significance of HOCl. In this work, a novel fluorescent probe based on a selenide switch for the detection of HOCl in lysosomes has been designed and synthesized on a 1,8-naphthalimide scaffold. The probe exhibited a high selectivity for HOCl over various reactive oxygen species (ROS) with a fast response and a large fluorescence enhancement in aqueous media. Confocal microscopy imaging of living cells indicated that the probe was able to accumulate in lysosomes and was successfully applied to imaging exogenous HOCl in living cells. Attempts of using Lyso-NI-Se to image HOCl in stimulated RAW264.7 cells failed, probably due to the absence of endogenous HOCl in lysosomes or the undesirable detection limit.

Keywords
Fluorescent probes; Naphthalimide; Lysosomes; Selenium; Hypochlorous acid
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Development of a selenide-based fluorescent probe for imaging hypochlorous acid in lysosomes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 299, 15 February 2015, Pages 1–8
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us