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Mitochondria-targeted oxygen probes based on cationic iridium complexes with a 5-amino-1, 10-phenanthroline ligand

Paper ID Volume ID Publish Year Pages File Format Full-Text
26409 43950 2015 11 PDF Available
Title
Mitochondria-targeted oxygen probes based on cationic iridium complexes with a 5-amino-1, 10-phenanthroline ligand
Abstract

•We developed mitochondria-targeted oxygen probes based on cationic iridium complexes.•The complexes are internalized into cells without using any transfection reagents.•They are preferentially distributed into mitochondria in living HeLa cells.•Phosphorescence of the complexes shows reversible oxygen response in living cells.

A cationic iridium(III) complex (btp)2Ir(phen-NH2) (btp = benzothienylpyridine; phen-NH2 = 5-amino-1,10-phenanthroline) and its analog with extended π-electronic structures (btq)2Ir(phen-NH2) (btq = benzothienylquinoline), (ttph)2Ir(phen-NH2) (ttph = thienothiophenylphenanthridine), (btph)2Ir(phen-NH2) (btph = benzothienylphenanthridine) have been designed and synthesized to develop new oxygen probes for living cells. The photophysical and cellular properties of these complexes were systematically investigated by using (btp)2Ir(acac) and its derivatives as reference compounds. The extension of the π-electronic systems of ligands resulted in remarkable red shifts in the absorption and phosphorescence spectra; the phosphorescence bands of (ttph)2Ir(phen-NH2) and (btph)2Ir(phen-NH2) appeared in the near-infrared region, maintaining the relatively high phosphorescence quantum yields (Φp0 = 0.14–0.24). Dimethylation and diethylation of the amino group of the phen-NH2 ligand in (btp)2Ir(phen-NH2) and its analog significantly enhanced the cellular uptake efficiency as compared with their neutral analog with an acetylacetonate (acac) ligand. The cationic iridium(III) complex (btp)2Ir(phen-NH2) and its analog internalized into living HeLa cells showed selective distribution into mitochondria. Owing to the high cellular uptake, relatively high cytotoxicity was found for the cationic iridium complexes. The phosphorescence intensity of newly developed iridium complexes exhibited oxygen response in HeLa cells, demonstrating that these complexes have potential as mitochondria-selective oxygen sensors for biological cells and tissues.

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Keywords
Iridium complex; Phosphorescence; Mitochondria; Oxygen probes; Living cells
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Mitochondria-targeted oxygen probes based on cationic iridium complexes with a 5-amino-1, 10-phenanthroline ligand
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 299, 15 February 2015, Pages 172–182
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us