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PEG-based hydrogels as an in vitro encapsulation platform for testing controlled β-cell microenvironments

Paper ID Volume ID Publish Year Pages File Format Full-Text
2642 121 2006 8 PDF Available
Title
PEG-based hydrogels as an in vitro encapsulation platform for testing controlled β-cell microenvironments
Abstract

An in vitro encapsulation platform for systematically testing the effects of microenvironmental parameters on encapsulated islets was developed. The base encapsulation matrix was a biocompatible hydrogel formed via the photoinitiated polymerization of dimethacrylated poly(ethylene glycol) (PEGDM). The resulting inert encapsulation matrix affords control over the biochemical and biophysical cellular microenvironment and the introduction of systematic changes to this environment. The compatibility of the PEG-based encapsulation platform with pancreatic β-cells was first established using a murine β-cell line, MIN6. When cell–cell contacts were introduced via aggregation of MIN6 β-cells prior to encapsulation, MIN6 β-cells remained viable within the PEG hydrogel platform throughout 3 weeks of in vitro culture. Proliferating cells were observed within encapsulated MIN6 aggregates qualitatively with bromodeoxyuridine staining and quantitatively by measuring the DNA content of encapsulation samples with time. MIN6 β-cells were encapsulated in hydrogels formed from three PEGDM macromers of varying molecular weights (M¯n=4000, 8000, 10,000 g/mol), and the resulting differences in hydrogel crosslinking density, which influences transport properties, did not affect encapsulated β-cell survival. Encapsulated MIN6 β-cells transplanted into diabetic mice returned blood glucose levels to normal levels, indicating in vivo function. Finally, the compatibility of the PEG encapsulation system with freshly isolated islets was confirmed.

Keywords
Islet encapsulation; Poly(ethylene glycol) hydrogels; β-Cell viability
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PEG-based hydrogels as an in vitro encapsulation platform for testing controlled β-cell microenvironments
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 2, Issue 1, January 2006, Pages 1–8
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us