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A new colorimetric and turn-on fluorescent chemosensor for Al3+ in aqueous medium and its application in live-cell imaging

Paper ID Volume ID Publish Year Pages File Format Full-Text
26430 43952 2016 6 PDF Available
Title
A new colorimetric and turn-on fluorescent chemosensor for Al3+ in aqueous medium and its application in live-cell imaging
Abstract

•A novel Schiff base fluorescent chemosensor HL was prepared.•Optical absorption and emission measurements were performed.•HL manifests selective signaling response to Al3+ in aqueous medium.•HL is sensitive to Al3+ with detection limit of 36.6 nM.•The application of HL for imaging of Al3+ in living cells was achieved.

A new simple Schiff-base ligand 2-Hydroxy-1-naphthaldehyde-(2-pyridyl) hydrazone (HL) was synthesized and characterized as a fluorescent probe. In aqueous solution containing 10% ethanol (Hexamethylenetetramine-HCl buffer, pH5.3), HL selectively binds Al3+to form a 1:1 ligand/metal complex, resulting in a color change from colorless to yellow–green and a significant fluorescence enhancement at 454 nm. The addition of EDTA quenches fluorescence of the HL·Al3+ complex, indicating that HL serves as a reversible chemosensor for Al3+. Under the optimum conditions, the dynamic range of the system was found to be linear up to 4.0 × 10−6 M Al3+ ions with a limit of detection of 36.6 nM. The probe is very effective for detection of intracellular Al3+ through fluorescence microscopic imaging.

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Keywords
Fluorescent chemosensor; Colorimetric; Al3+; Naphthalene; Cell imaging; Aqueous medium
First Page Preview
A new colorimetric and turn-on fluorescent chemosensor for Al3+ in aqueous medium and its application in live-cell imaging
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 315, 15 January 2016, Pages 8–13
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering