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Enhanced extracellular matrix production and differentiation of human embryonic germ cell derivatives in biodegradable poly(ε-caprolactone-co-ethyl ethylene phosphate) scaffold

Paper ID Volume ID Publish Year Pages File Format Full-Text
2658 122 2006 12 PDF Available
Title
Enhanced extracellular matrix production and differentiation of human embryonic germ cell derivatives in biodegradable poly(ε-caprolactone-co-ethyl ethylene phosphate) scaffold
Abstract

Extracellular environment regulates cell behavior and also influences the differentiation of stem cells. Two cell lines of pluriopotent human embryonic germ cell derivatives (EBD cells) were cultured on a biodegradable poly(ε-caprolactone-co-ethyl ethylene phosphate) (PCLEEP) and non-degradable cellulose acetate scaffold. Their cell behaviors including proliferation, differentiation, cell distribution and extracellular matrix production were studied for 4 weeks and 10 months. The proliferation of the EBD cells was enhanced in both of the three-dimensional scaffolds in the first 5 weeks of culture, regardless of the material difference, compared to monolayer culture. While the gene expression profile remained multilineage for the EBD cells cultured in the cellulose acetate fibrous scaffold, much of the neuronal lineage markers were down-regulated in EBD cells cultured in the PCLEEP scaffold. On the other hand, extracellular matrix production was significantly enhanced in the PCLEEP scaffold. The study showed that the polymer substrate could influence the differentiation and growth of pluripotent stem cells in the absence of exogenous biochemical signals.

Keywords
Polymer scaffold; Biodegradability; Stem cells; Extracellular matrix; Differentiation
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Enhanced extracellular matrix production and differentiation of human embryonic germ cell derivatives in biodegradable poly(ε-caprolactone-co-ethyl ethylene phosphate) scaffold
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 2, Issue 4, July 2006, Pages 365–376
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us