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Steady-state and time-resolved spectroscopic studies of green-to-red photoconversion of fluorescent protein Dendra2

Paper ID Volume ID Publish Year Pages File Format Full-Text
26614 43967 2014 9 PDF Available
Title
Steady-state and time-resolved spectroscopic studies of green-to-red photoconversion of fluorescent protein Dendra2
Abstract

•Green-to-red photoconversion (PC) of fluorescent protein Dendra2 was studied.•CW and double-pulsed excitation with multiple wavelengths was used.•PC occurs following the absorption of one photon without intermediate states involved.•Both neutral and anionic forms of the green chromophore can undergo PC.

The excited state dynamics and green-to-red photoconversion (PC) of the monomer fluorescent protein Dendra2 were studied using time resolved pump-probe and double-pulsed excitation experiments. A linear dependence of the PC efficiency on the pump power is demonstrated when exciting the neutral green form at 400 nm as well as anionic green form at either 458 or 488 nm. Rather large PC quantum yields of ∼1.5 × 10−3 for the neutral form and ∼6 × 10−5 for the anionic form are determined from exposure-dependent PC saturation experiments. At the same time, no dependence of the PC rate on the time delay between the pulses is observed, as well as no significant difference between PC via pulsed or continuous-wave (CW) excitation. Our wavelength- and pH-dependent studies of the PC process suggest that the Dendra2 green-to-red conversion occurs following the absorption of one photon, directly from the excited state of each (neutral and anionic) form of the green protein without intermediate excited states or species requiring absorption of additional photons.

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Keywords
Fluorescent Proteins; Photoconversion; Dendra2; Transient absorption
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Steady-state and time-resolved spectroscopic studies of green-to-red photoconversion of fluorescent protein Dendra2
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 280, 15 April 2014, Pages 5–13
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us