fulltext.study @t Gmail

Uptake of C60 by human monocyte macrophages, its localization and implications for toxicity: Studied by high resolution electron microscopy and electron tomography

Paper ID Volume ID Publish Year Pages File Format Full-Text
2662 122 2006 11 PDF Available
Title
Uptake of C60 by human monocyte macrophages, its localization and implications for toxicity: Studied by high resolution electron microscopy and electron tomography
Abstract

Despite great interest in the engineering applications of carbon-based nanoparticles, recent studies have raised concerns about their potential toxicity and safety. The release of C60 into the environment has been suggested to be a potential risk with possible ecological implications. Here we evaluate energy-filtered transmission electron microscopy (EFTEM) and scanning transmission electron microscopy (STEM)-based electron tomography as techniques for imaging the three-dimensional (3-D) distribution of nanoparticles within cells. Our aim was to establish if human monocyte macrophages internalise nanoparticles and to assess whether nanoparticles are modified by cells following uptake. Using these techniques we were able to show a marked increased in the amount of information gained from 3-D imaging. 3-D electron tomography revealed several sub-cellular compartments containing C60 within the cell: secondary lysosomes, along the outer and nuclear membrane and most notably inside the nucleus of the cell. Using EFTEM and STEM-based techniques we were able to visualize cell structures such as membranes, the mitochondria, ribosomes and the nucleus, without the need for traditional staining techniques. In particular we demonstrate the potential of electron tomography for whole cell studies to enable 3-D distributions of particles within cells. The concentrations of C60 used in this study were not toxic and were chosen to study which sub-cellular compartments accumulated C60. Knowledge of the sites of accumulation of nanoparticles will allow us to predict vulnerability if the nanoparticles can generate free radicals.

Keywords
3-D electron tomography; Buckminster fullerene; Toxicity; Intracellular distributions
First Page Preview
Uptake of C60 by human monocyte macrophages, its localization and implications for toxicity: Studied by high resolution electron microscopy and electron tomography
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 2, Issue 4, July 2006, Pages 409–419
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us